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. 2021 Apr 22;10:e65435. doi: 10.7554/eLife.65435

Figure 1. TG6, F5, and OT-1 CD8SP cells express CD5 and Nur77 across the polyclonal spectrum.

CD8SP cells were harvested from the thymus and lymph nodes of wild-type (B6), TG6, F5, and OT-1 TCRtg mice and analyzed by flow cytometry. (a, b) Representative (left) and quantified by geometric mean fluorescence intensity (right) CD5 surface expression on (a) CD8SP thymocytes and (b) CD8SP lymph node cells. (c, d) Representative (left) and quantified (right) intracellular Nurr77 expression gated on (c) CD8SP thymocytes and (d) CD8SP lymph node cells. Data are presented as average ± SD and analyzed using an ordinary one-way ANOVA followed by a Tukey’s multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). All data are compiled from three or more experiments.

Figure 1.

Figure 1—figure supplement 1. TG6 mice have efficient positive selection and allelic exclusion in the thymus.

Figure 1—figure supplement 1.

Thymuses from B6xB6C (H2b/d), TG6 H2b/d Rag+/+, and TG6 H2b/d Rag−/− mice were harvested and analyzed via flow cytometry. (a) Total thymocyte numbers. (b) Representative flow plots and quantification of the percent of DP, CD4SP, and CD8SP thymocytes for the indicated genotypes. (c) Allelic exclusion measured by percent TCRβ and Vα2, Vβ8, or Vβ2 double-positive out of CD8SP thymocytes from the indicated genotypes. Data are compiled from two experiments.