Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mouse) | Gad2-IRES-Cre | The Jackson Laboratory | JAX: 010802 (RRID:IMSR_JAX:014548) |
Cre targeted at theGad2 locus |
| Strain, strain background (Mouse) | PV-IRES-Cre | The Jackson Laboratory | JAX: 008069 (RRID:IMSR_JAX:008069) |
Cre targeted at thePvalb locus |
| Strain, strain background (Mouse) | VGAT-ChR2-EYFP | The Jackson Laboratory | JAX: 014548 (RRID:IMSR_JAX:014548) |
ChR2 targeted at theSlc32a1 locus |
| Strain, strain background (Mouse) | Ai32(RC-hR2(H134R)/EYFP) | The Jackson Laboratory | JAX: 012569 (RRID:IMSR_JAX:012569) |
ChR2 targeted at theGt(ROSA)26Sor locus |
| Recombinant DNA reagent | AAV9-hSyn-FLEX-ChrimsonR-tdTomato | UNC Viral Core | N/A | |
| Recombinant DNA reagent | AAV8-Ef1a-DIO-ChRmine-mScarlet-WPRE | Stanford Viral Core | GVVC-AAV-188 | |
| Recombinant DNA reagent | AAV-pCAG-FLEX-EGFP-WPRE | Addgene | 51502 (RRID:Addgene_51502) |
|
| Software, algorithm | MATLAB | Mathworks | https://www.mathworks.com | |
| Other | Design files, software, and documentations for the automated home-cage system. | This paper - Github repository | https://github.com/NuoLiLabBCM/Autocage | The Github repository contains the hardware design files and software for the construction of automated home-cage system, along with documentations and protocols for automated head-fixation training and task training. |