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. 2021 Apr 27;10:e65537. doi: 10.7554/eLife.65537

Figure 5. Complex FPA-dependent patterns of alternative polyadenylation at RPP4.

FPA-dependent intronic, exonic and readthrough poly(A) site selection in RPP4. (Inset 1) A magnified view of TIR-domain-only RPP4 transcripts detected in 35S::FPA:YFP caused by proximal polyadenylation in intron 1, and distal polyadenylation within the TE associated with cryptic splicing. (Inset 2) A magnified view of the stop-codonless transcripts produced within the protein-coding RPP4 region in fpa-8.

Figure 5.

Figure 5—figure supplement 1. Complex FPA-dependent patterns of alternative polyadenylation at the RPP4 locus.

Figure 5—figure supplement 1.

Comparison of the expression of four classes of RPP4 (AT4G16860) transcripts detected using (A) Nanopore DRS or (B) Helicos DRS. N-terminal truncation, TIR-domain-only transcripts generated by proximal intronic polyadenylation or distal polyadenylation and cryptic splicing; Non-stop, mRNAs lacking in-frame stop codons; Full-length, full-length protein-coding mRNAs; and Chimeric, mRNAs containing RPP4, COPIA-like retrotransposon (AT4G16870) and/or downstream AT4G16857.