Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain (Arabidopsis thaliana) | Columbia (Col-0) | NA | ABRC: CS22625 | Country of Origin: USA |
| Strain (Arabidopsis thaliana) | Keswick (Ksk-1) | Lai and Eulgem, 2018 | ABRC: CS1634 | Country of Origin: UK |
| Gene (Arabidopsis thaliana) | FPA | NA | TAIR/ABRC: AT2G43410 | - |
| Gene (Arabidopsis thaliana) | RPP7 | NA | TAIR/ABRC: AT1G58602 | - |
| Genetic reagent (Arabidopsis thaliana) | fpa-7 | Duc et al., 2013 | ABRC: SALK_021959C | T-DNA insertion mutant in Col-0 background. Gifted by R. Amasino, UW-Madison. |
| Genetic reagent (Arabidopsis thaliana) | fpa-8 | Bäurle et al., 2007 | TAIR: 4515120225 | EMS point mutation in Col-0 background. Gifted by C. Dean, John Innes Centre |
| Genetic reagent (Arabidopsis thaliana) | 35S::FPA:YFP fpa-8 | Bäurle et al., 2007 | NA | Transgenic line in fpa-8 background, gifted by C. Dean, John Innes Centre |
| Genetic reagent (Arabidopsis thaliana) | pFPA::FPA fpa-8 | Zhang et al., 2016 | NA | Transgenic line in fpa-8 background. |
| Genetic reagent (Arabidopsis thaliana) | vir-1 | Růžička et al., 2017 | TAIR: 6532672723 | EMS point mutant in Col-0 background. Gifted by K. Růžička, Brno. |
| Commercial assay, kit | Rneasy Plant Mini kit | QIAGEN | Cat#: 74904 | - |
| Commercial assay, kit | SuperScript III Reverse Transcriptase | Thermo Fisher Scientific | Cat#: 18080044 | - |
| Commercial assay, kit | NEBNext Ultra Directional RNA Library Prep Kit for Illumina | New England Biolabs | Cat#: E7420 | - |
| Commercial assay, kit | Dynabeads mRNA Purification Kit | Thermo Fisher Scientific | Cat#: 61006 | - |
| Commercial assay, kit | Nanopore Direct RNA sequencing kit | Oxford Nanopore Technologies | Cat#: SQK-RNA001 | - |
| Commercial assay, kit | MinION Flow cell r9.4 | Oxford Nanopore Technologies | Cat#: FLO-MIN106 | - |
| Peptide, recombinant protein | T4 DNA ligase | New England Biolabs | Cat#: M0202 | - |
| Commercial assay, kit | Quick Ligase reaction buffer | New England Biolabs | Cat#: B6058S | - |
| Commercial assay, kit | Agencourt RNAclean XP magnetic beads | Beckman Coulter | Cat#: A63987 | - |
| Commercial assay, kit | Qubit RNA BR Assay Kit | Thermo Fisher Scientific | Cat#: Q10210 | - |
| Commercial assay or kit | RNA ScreenTape System | Agilent | Cat#: 5067–5576 - 5067–5578 | - |
| Antibody | FPA antibody | Covance | NA | Rabbit polyclonal antibody. Raised against FPA amino acids536–901. |
| Chemical compound | [γ−32P]-ATP | Perkin Elmer | Cat#: BLU012H250UC | - |
| Commercial assay or kit | DECAprime II DNA labelling kit | Thermo Fisher Scientific | Cat#: AM1455 | - |
| Commercial assay or kit | Illustra MicroSpin G-50 Columns | GE Healthcare | Cat#: 27-5330-01 | - |
| Commercial assay or kit | RiboRuler High Range RNA Ladder | Thermo Fisher Scientific | Cat#: SM1821 | - |
| Peptide, recombinant protein | FastAP Thermosensitive Alkaline Phosphatase | Thermo Fisher Scientific | Cat#: EF0651 | - |
| Peptide, recombinant protein | T4 Polynucleotide Kinase | Thermo Fisher Scientific | Cat#: EK0031 | - |
| Peptide, recombinant protein | Nuclease P1 | Merck | Cat#: N8630-1VL | - |
| Peptide, recombinant protein | Calf Intestinal Alkaline Phosphatase | New England Biolabs | Cat#: M0290S | - |
| Chemical compound | N6-Methyladenosine (m6A), Modified adenosine analog | Abcam | Cat#: ab145715 | - |
| Chemical compound | Adenosine, Endogenous P1 receptor agonist | Abcam | Cat#: ab120498 | - |
| Commercial assay or kit | GFP-Trap Agarose | Chromotek | Cat#: gta-20 | - |
| Software, algorithm | d3pendr | 10.5281/zenodo.4319112 | NA | Scripts to perform differential 3' end analysis using Nanopore DRS or Helicos DRS data |
| Software, algorithm | Simpson_Barton_FPA_NLRs | 10.5281/zenodo.4319108 | NA | All pipelines, scripts and notebooks used for analyses in this manuscript. |