Figure 1. Characterization of isolated MSCs. (a) Morphological characterization. After the fourth passage, MSCs appeared as homogeneous, spindle-shaped, fibroblast-like cells (200× magnification). (b) Expression of immunophenotypic surface markers. Graphs display the phenotype of MSCs in culture, after the fourth passage; CD90 (99.9%), CD105 (95.9%), CD73 (99.2%), and Lin (2.0%). (c) In vitro osteogenic differentiation test. MSCs were able to differentiate towards osteogenic lineages, which appeared as red color in most MSC populations, following Alizarin Red staining (200× magnification).