Table 1.
Primer sequences used for real-time PCR reactions.
| Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Amplicon length |
|---|---|---|---|
| 18s | CTTTGCCATCACTGCCATTAAG | TCCATCCTTTACATCCTTCTGTC | 199 bp |
| IL-6 | GTACATCCTCGACGGCATC | ACCTCAAACTCCAAAAGACCAG | 198 bp |
| TNF-α | CCTTCCTGATCGTGGCAG | GCTTGAGGGTTTGCTACAAC | 184 bp |
| IL-8 | GTGTAAACATGACTTCCAAGCTG | GTCCACTCTCAATCACTCTCAG | 182 bp |
| iNOS | GGTATCCTGGAGCGAGTGGT | CTCTCAGGCTCTTCTGTGGC | 212 bp |
| COX-2 | GACAGTCCACCAACTTACAATG | GGCAATCATCAGGCACAGG | 105 bp |
| nAChRα7 | CTGCTCGTGGCTGAGATCAT | CTGGTCCACTTGGGCATCTT | 167 bp |