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Concentrations of primary antibodies used for western blot.
| Antibody | Dilution (μl) | |
|---|---|---|
| TNFα | Cell Signaling Technology; cs 11948S | 1/1000 |
| Collagen I | Santa Cruz, sc 293182 | 1/100 |
| Collagen III | Santa Cruz, sc 271249 | 1/100 |
| AMPKα | Cell Signaling Technology; cs 2532S | 1/1000 |
| PPARα | Santa Cruz; sc-9000 (H-98) | 1/100 |
| Phosphorylated AKT | Cell Signaling Technology (Ser473); cs 4060S | 1/2000 |
| AKT | Cell Signaling Technology; cs 9272 | 1/1000 |
| Top2-β | Abcam; ab109524 | 1/10000 |
| GAPDH | Santa Cruz; sc 32233 | 1/10000 |
