Table 3.

Primer sequences used for bacteria 16S rRNA.

Bacteria	Sequence 5′-3′	Length (bp)	Reference	Annealing temp, °C
Total bacteria	F: GTGSTGCAYGGYYGTCGTCA	123	[26]	60
	R: ACGTCRTCCMCNCCTTCCTC
Bacteroidetes	F: GGARCATGTGGTTTAATTCGATGAT	126	[27]	60
	R: AGCTGACGACAACCATGCAG
Firmicutes	F: GGAGYATGTGGTTTAATTCGAAGCA	126	[27]	60
	R: AGCTGACGACAACCATGCAC
Bifidobacterium	F: TCGCGTCYGGTGTGAAAG	128	[28]	62
	R: GGTGTTCTTCCCGATATCTACA
Clostridium cluster IV	F: GCACAAGCAGTGGAGT	240	[29]	54
	R: CTTCCTCCGTTTTGTCAA
Escherichia coli	F: CATGCCGCGTGTATGAAGAA	95	[30]	62
	R: CGGGTAACGTCAATGAGCAAA
Lactobacillus	F: AGCAGTAGGGAATCTTCCA	345	[31]	62
	R: ATTCCACCGCTACACATG