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Parasitism in L. major- or L. amazonensis-infected macrophages upon IGF-I siRNA transfection. RAW 264.7 cells infected with L. major or L. amazonensis promastigotes transfected with or without 150 μM IGF-I siRNA for 6 hours. The parasitism (median number of parasites per 100 cells) was evaluated after 24 and 48 hours. One representative experiment from three independent assays is shown. ∗p < 0.05 (ANOVA and Tukey's tests).
