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. 2021 May 4;2021:6614475. doi: 10.1155/2021/6614475

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Copyright © 2021 Luiza C. Reis et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Parasitism in response to cytokine treatments and IGF-I siRNA transfection. Parasitism (median number of parasites per 100 cells) in L. amazonensis-infected RAW 264.7 cells transfected with or without 150 μM IGF-I siRNA for 6 hours and then were stimulated with IFN-γ (200 U/mL; R&D Systems, EUA), IL-4 (2 ng/mL; R&D Systems, EUA), or IL-13 (5 ng/mL; R&D Systems, EUA) for 24 (a) and 48 (b) hours. One representative experiment from three independent assays is shown. ^∗p < 0.05 (ANOVA and Tukey's tests).