Table 4.
A summary of studies investigating thermal inactivation treatments for HEV in non-food matrix samples
| Study | Cell culture or molecular detection? | Genotype | Heat treatment | Growth period | Inactivation/reduction | |
|---|---|---|---|---|---|---|
| Temperature (°C) | Time (min) | |||||
| Emerson et al. (2005) | Cell culture, HepG2/C3A cells | 1 (strain Akluja) | 56 | 60 | 5–6 days | > 80% reduction |
| 1 (strain SAR55b) | 56 | 60 | 5–6 days | ~ 50% reduction | ||
| 2 (strain Mex14c) | 60 | 60 | 5–6 days | 96% reduction | ||
| Huang et al. (1999) | Cell culture, A549 cells | 3 (strains G93-1*, G93-2*, G93-3*, G93-4*) | 56 | 30 | 72 h | < 1.0 (TCID50/0.025 ml) |
| Johne et al. (2016) | Cell culture, A549/D3 cells | 3 (strain 47832cf) | 55 | 1 | 35 days | ~ 1 log reduction in focus forming units |
| 70 | 2 | “No infectious virus” | ||||
| Schielke et al. (2011) | Molecular detection | 3 (strain wbGER27e) | 56 | 15 | N/A | 74.07% reduction |
| 3 (strain wbGER27e) | 56 | 60 | N/A | 99.90% reduction | ||
| Tanaka et al. (2007) | Cell culture, PLC/PRF/5 cells | 3 (strain JE03-1760Fd) | 70 | 10 | 35 days | “No infectious virus” |
| 3 (strain JE03-1760Fd) | 56 | 30 | 50 days | “Still infectious” | ||