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. 2021 May 13;11:9966. doi: 10.1038/s41598-021-88568-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of cold storage on whitespotted bamboo shark Chiloscyllium plagiosum ejaculates. Plasma membrane integrity (a) and motility (b) of ejaculates cold-stored raw or extended with elasmobranch modified Hank’s balanced salt solution (E-HBSS) or artificial seawater (ASW) and initial mean (dotted line) and standard error (grey rectangle) of ejaculates. Treatment bars are capped with a circle (estimated marginal mean) and error bars (95% confidence interval). *Indicates significant difference among treatments at each time point (p < 0.05).