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. 2021 May 13;12(5):479. doi: 10.1038/s41419-021-03763-z

Fig. 2. YTHDF2 regulated LUAD cell proliferation and viability in vitro and vivo.

Fig. 2

a Western blot analysis of YTHDF2 expression in A549 and H1792 cells infected with two independent shRNAs targeting YTHDF2 or a control shRNA. b Trypan blue live cell count assays were performed to determine cell growth after YTHDF2 knockdown in A549 and H1792 cells. c Western blot analysis for YTHDF2 expression in A549 and H1792 cells that were lentivirally infected with YTHDF2 or a control vector. d Trypan blue live cell count assays were performed to determine cell growth after YTHDF2 was over-expressed in A549 and H1792 cells. e Colony formation assays of A549 and H1792 cells described in a. f Colony formation assays of A549 and H1792 cells described in c. A549 cells with empty vector or YTHDF2 knockdown vectors were subcutaneously injected into nude mice. h Tumor size was measured every 3 days, and growth curves were plotted. Tumors were dissected from nude mice of each group and photographed at 32 days after transplantation, and the size and weight of tumors were measured (g, i). All data and error bars are presented as the mean ± SDs. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with control cells.