Fig. 5.

Drug treatments have no effect on myelin degradation or oligodendrocytes in the lesion. (A–D) Representative images of treatment groups from the day 3 lysolecithin lesion, visualizing Olig2⁺ cells (white), NFH⁺ axons (green; see Fig. 7), MBP⁺ myelin (red), and the overlay image. (E) Number of oligodendrocyte lineage cells in the lesion. (F) Comparisons of MBP⁺ staining within the lesion, indicative of myelin debris. Pooled data from the three separate experiments (n ≥ 5 each), normalized and represented as fold change of the vehicle group. Graphs represented are the mean with error bars depicting SEM. Data were analyzed using a one-way ANOVA with Dunnett’s post hoc multiple comparison test, compared to vehicle treatment. Each data point represents one animal. Horizontal dashed line represents fold change of 1 (the mean of vehicle group). IND: indapamide, HCQ: hydroxychloroquine