Fig. 1.

Phosphoproteomics profiling of DRG. Paclitaxel (PAC, 2 mg/kg, n = 3) or vehicle (4% dimethyl sulfoxide and 4% Tween 80 in saline, 1 ml/kg, n = 3) was injected intraperitoneally on days 0, 2, 4, and 6 in the PAC and vehicle groups. (a) Overall scheme describing sample preparation, phosphor-proteome profiling, and data analysis. On day 16 after the first PAC or vehicle injection, the L1-6 DRG were obtained, homogenized, lysed, digested with trypsin, and labeled with TMT. TMT-labeled peptides were fractioned, immobilized, enriched with metal-affinity chromatography phosphopeptide, and analyzed with LC-MS/MS systems. DR, dorsal root; VR, ventral root; DH, dorsal horn; and VH, ventral horn. (b) Of the 22,701 phosphopeptides identified, 2416 were differentially expressed, with statistical significance (n = 3, p ≤ 0.1). (c) Volcano plot for all phosphorylated proteins (n = 3). (d) Enrichment plot of the Wnt signaling pathway (n = 3). (e) Phosphorylated sites of canonical Wnt signaling-related proteins (n = 3). (f) The graph shows the position of the phosphorylated GSK3β