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. 2020 Nov 16;18(1):412–429. doi: 10.1007/s13311-020-00958-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Localization of HuR protein in activated microglia from EAE spinal cord. Fluorescence microscopy images showed the microglial localization of HuR in the spinal cord of control (CTRL), EAE, or EAE + anti-HuR ASO mice. a, c, e: Low-magnification images show a homogeneous distribution of HuR within the spinal cord. Immunostaining for the microglial marker CD11b in the same section reveals similar immunoreactivity. The merged image shows HuR localization on microglial cells. b, d, f: High-magnification images show the cytosolic localization of HuR, consistent with a pattern of activation, and merged images illustrate the high degree of colocalization of HuR and CD11b. Nuclei were counterstained with DAPI. Antibodies are shown at the top. Scale bar = 50 μm. Expression of HuR protein on TMEM119-positive microglial cells. Fluorescence microscopy images showed the microglial localization of HuR in the spinal cord of control (CTRL), EAE, or EAE + anti-HuR ASO mice. g, i, k: low-magnification images. h, j, l: high-magnification images. Antibodies are shown at the top. Evaluation of colocalization of HuR and CD11 (m) or TMEM119 (n) in spinal cord sections. Scale bars = 100 μm. CTRL, dODN-treated nonimmunized mice; EAE, dODN-treated EAE mice; EAE + ASO, anti-HuR ASO-treated EAE mice