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. 2020 Nov 16;18(1):412–429. doi: 10.1007/s13311-020-00958-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Anti-HuR ASO activated the anti-inflammatory microglial phenotype. (a) Representative microphotographs showed a decreased CD206 immunostaining in the spinal cord section of EAE mice on day 30 postimmunization. The CD206 protein expression was restored by HuR silencing. Merged images showed the expression of HuR on CD206-expressing cells. Quantification analysis of immunofluorescence (b) data showed the significant reduction of CD206 immunostaining, reversed by HuR silencing. (c) Evaluation of colocalization of HuR and CD206 in spinal cord sections. (d) Western blot results showing anti-HuR ASO reversal of EAE-induced reduction of CD206 protein expression. (e) Lack of variation of IL-10 levels in EAE mice in the absence or in the presence of HuR silencing (Western blot). (f) Representative blots of Western blotting experiments. CTRL, dODN-treated nonimmunized mice; EAE, dODN-treated EAE mice; EAE + ASO, anti-HuR ASO-treated EAE mice. Error bars represent the standard error of the mean. Antibodies are shown at the top. Scale bar = 100 μm. *p < 0.05, **p < 0.01 versus CTRL; °p < 0.05 versus EAE (ANOVA)