Fig. 2.

Microarray chip spotting and assembly. a Schematic overview of antigen immobilization strategies, with DSC and diepoxy PEG immobilization being two-step processes (chip surface activation followed by antigen immobilization) and EDC/s-NHS as one-step process (antigen activation in spotting solution), antigens shown in blue, immobilization is done via amino or carboxy groups of the amino acid side chains. b Chip assembly from carrier (top), adhesive foil with flow channels (middle), and glass microarray chip (bottom). c Photograph of an assembled chip