Table 2.
Main assay steps on the MCR 3 with details to used volumes and flow rates; a video showing the measurement process is provided in the Supplementary Information
| Step | Volume | Flow rate | |
|---|---|---|---|
| Sample injection | 900 μL | 10 μL s−1 | |
| Flushing |
1000 μL 2000 μL |
10 μL s−1 500 μL s−1 |
|
| Detection antibody injection |
200 μL 800 μL |
100 μL s−1 10 μL s−1 |
|
| Flushing |
1000 μL 2000 μL |
10 μL s−1 500 μL s−1 |
|
| CL reagents injection | 400 μL | 150 μL s−1 | |
| Image acquisition | - | - | |
| Flushing of whole system |
11 mL 8 mL |
250 μL s−1 500 μL s−1 |
(flushing of sample syringe) (flushing of tubes and chip) |