Fig. 2.
Levistolide A decreased the Aβ generation and deposition and reduced the expression of phosphorylation of tau protein and loss of synapses in APP/PS1 Tg mice. (a, b) Quantitative analysis of Aβ immunohistochemistry staining in the brains of APP/PS1 Tg mice. (c, d) Expression levels of Aβ oligomer protein in the cortex tissue of APP/PS1 Tg mice. β-actin was used as internal control. (e, f) Expression levels of Aβ1–40 and Aβ1–42 in the serum of APP/PS1 Tg mice. (g, h) Expression levels of Aβ1–40 and Aβ1–42 in the cortex tissue of APP/PS1 Tg mice. (i) Western blots showing the protein levels, including total tau, p-tau (Thr181, Ser396, Ser416), SYP, and PSD95. GAPDH was used as internal control. (j–o) Quantitative analysis of the results shown in i. Data represent the mean ± SD (n > 3), *p < 0.05, **p < 0.01, compared with the vehicle-treated APP/PS1 Tg mice