Fig. 3.

Levistolide A mitigated activation of Aβ-accompanied glial cells in APP/PS1 Tg mice. (a) Double immunofluorescence staining analyzed the distribution and expression of Aβ with GFAP and Iba-1 in cortex and hippocampus of APP/PS1 Tg mice brains. (b) Western blot detection of GFAP, Iba-1, IL-6, IL-1β, and TNF-α in the cortex of APP/PS1 Tg mice. β-actin was used as internal control. (c–g) Quantitative analysis of the immunoreactivities to the antibodies presented in the previous panel. Data represent the mean ± SD (n > 3), *p < 0.05, **p < 0.01, compared with the vehicle-treated APP/PS1 Tg mice