Fig. 4.
Levistolide A treatment accelerated the nonamyloidogenic APP pathway in APP/PS1 Tg mice and in N2a/APP695swe cells. (a) Western blots showing the protein levels associated with APP metabolism, including sAPPα, ADAM10, TACE, sAPPβ, BACE1, PS1, PS2, and NCT. β-actin and GAPDH were used as internal control. (b–i) Quantitative analysis of the results shown in (a). (j) Cell viability was measured by MTT assay and expressed relative to the vehicle. (k, l) Expression levels of Aβ1–40 and Aβ1–42 in the supernatant and lysis of N2a/APP695swe cells. (m) Western blots showing the protein levels associated with APP metabolism, including Aβ oligomer, sAPPα, ADAM10, TACE, sAPPβ, BACE1, PS1, PS2, and NCT. β-actin and GAPDH were used as internal control. (n–v) Quantitative analysis of the results shown in (m). Data represent the mean ± SD (n > 3), *, #p < 0.05, **, ##p < 0.01, ***, ###p < 0.001, compared with the vehicle