Fig. 6.

Levistolide A promoted the activation of PPARγ to regulate APP processing. (a, b) Western blot showing the protein level of PPARγ in the cortex of APP/PS1 Tg mice. β-actin was used as internal control. (c, d) Western blot showing the protein level of PPARγ in the N2a/APP695swe cells. GAPDH was used as internal control. (e) Double-stained immunofluorescence of PPARγ and nuclei in N2a/APP695swe cells, the green fluorescence stands for PPARγ and the blue represents the nuclei. (f) Western blots showing the protein levels including ADAM10, TACE, BACE1, PS1, PS2, NCT, PPARγ, GSK-3α/β, and p-GSK-3α/β. GAPDH was used as internal control. (g–n) Quantitative analysis of the results shown in (f). Data represent the mean ± SD (n > 3), *, #p < 0.05, **, ##p < 0.01, ***, ###p < 0.001, compared with the vehicle control