Figure 1.

EV purification and characterization

(A) Isolation of fractions F1–F4 from conditioned media. (B) Gradient image after 150,000 × g centrifugation highlighting F1–F4. (C) Density profile of blank iodixanol gradient after 150,000 × g centrifugation. (D) Representative transmission electron micrographs of F1–F4. (E) Cholesterol, DNA, protein, and particle concentrations in F1–F4. Data from 3 EV isolations are plotted as mean ± SD (F) Representative protein normalized SDS-PAGE of F1–F4, producer cell lysate (CL), and crude ultracentrifuged (UC) pellet with immunoblots for individual markers. Molecular weight markers for all immunoblots and SDS-PAGE gels are given in kDa. (G) Heatmap plotting quantitative peptide spectrum matches for proteins indicated. Proteins highly enriched in F1 (top) and F4 (bottom) are shown. Data are averaged from triplicate measurements from a representative EV isolation.