Figure 1.

Viral replication and oncolytic effect in MDRVV and FUVAC

(A) Schematic representation of MDRVV and FUVAC. (B) A549 cells were infected with MDRVV- or FUVAC-LG/DsRed at an MOI of 0.01, 0.1, or 1, and the images were taken 3 days after infection. Scale bar, 500 μm. (C) Viability of the A549 cells 72 h after virus infection as described in (B) was examined with CellTiter 96 Aqueous Nonradioactive Cell Proliferation Assay (Promega). Data are represented as the percent survival of mock-infected cells. (D) A549 cells infected with MDRVV- or FUVAC-LG/DsRed at an MOI of 0.1 were harvested after infection for 24, 48, or 72 h, and the extracted intracellular viruses were titrated in RK13 cells. Data in (C) and (D) are presented as the means ± SEM (n = 3). ∗∗∗p < 0.001 (two-tailed unpaired t test).