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. 2021 Apr 29;9:672693. doi: 10.3389/fcell.2021.672693

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Copyright © 2021 Yang, Lu, Mei, Sun, Han, Qian, Liang, Pan, Kong, Xu, Liu, Gao, Qi, Shou, Chen, Cao, Zhao, Lin, Zhao, Geng, Ma and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of autophagy and lysosome of irradiated MCF-7 cells and MCF-7 CSCs. (A) Compared with the control, the increasement of autophagy proportion for irradiated group. (B) Compared with the control, the increasement of lysosome for irradiated group. (C,D) Representative images of irradiated MCF-7 cells and MCF-7 CSCs stained with MDC autophagy detection kit and lysoTracker Red DND-99. The blue was the nucleus, the red was the lysosome, and the green labeled autophagy. Scale bars: 50 μm. *P < 0.05.