Table 1. .
Glycan specificity of plant/fungal agglutinins used for histochemical glycophenotyping
| Species | Abbreviation | Monosaccharide specificity | Potent glycan ligands |
|---|---|---|---|
| Arachis hypogaea (peanut) | PNA | Gal | Galβ3GalNAcα/β (in α-anomeric linkage to Ser/Thr known as core 1 disaccharide, Thomsen-Friedenreich antigen and CD176) |
| Erythrina cristagalli (coral tree) | ECA | Gal | Galβ4(3)GlcNAc(type II > type I), affinity increase by bivalency of Manβ2/6(LacNAc)2 |
| Lycopersicon esculentum (tomato) | LEA | a | Core and stem regions of high-mannose-type N-glycans, LacNAc oligomers (GlcNAcβ3Galβ4GlcNAcβ3Gal)n present in complex-type N-glycans and core 2/4 mucin-type O-glycansb, affinity increasing in proportion to chain lengthc |
|
Phaseolus vulgaris (leukoagglutinin) (kidney bean) |
PHA-L | a | Tetra- and tri-antennary N-glycans with β6-branching |
| Maackia amurensis (leukoagglutinin) | MAA-I | a | Neu5Acα3Galβ4Glc(NAc), 3'-sulfation tolerated |
| Polyporus squamosus (polypore mushroom) | PSL | a | Neu5Acα6Galβ4(3)Glc(NAc) (over 300-fold more active than LacNAc, not reactive with free Neu5Ac); 6'-sulfated LacNAc about 20-fold less active; sialyl Tn not active |
| Dolichos biflorus (horse gram) | DBA | GalNAc | GalNAcα3GalNAcα3Galβ4Galβ4Glc (αGalNAc (Tn) comparatively weak), histo-blood group A-tetrasaccharide |