Table 3. .
Immunohistochemical profiling of CG presence during embryogenesis of eye lens and two post-hatch ages
| Stageb/cell type | CG | ||||||
|---|---|---|---|---|---|---|---|
| CG-1A | CG-1B | CG-2a | CG-3 | CG-8 | C-GRP | C-GRIFINa | |
| HH stage 19 (ca. 68–72 h) | |||||||
| anterior epithelium of the lens vesiclec | ++ | −/(+) | +/++++h | (+)/+ | −/(+) | +++ | (+) |
| posterior part of the lens vesicle | ++ | −/(+) | + | − → + | −/(+) | +++ | +/++ |
| presumptive annular padd | +/++ | −/(+) | +/++++h | (+) | −/(+) | +++ | (+) |
| HH stage 23 (ca. 3.5–4 days) | |||||||
| anterior epithelium of the lens vesiclec | ++ | −/(+) | +/+++h | (+)/+ | −/(+) | +++ | − |
| primary lens fiber cells | +/++ | −/(+) | + | (+)/++ | −/(+) | +++ | +++ |
| presumptive annular padd | +/++ | −/(+) | +/++h | −/+ | −/(+) | +++ | − |
| HH stage 31 (ca. 7 days) | |||||||
| central anterior epithelial cells | ++/+++ | − | +/+++h | ++/+++ | +/++ | +++ | − |
| equatorial epithelial cellse | ++/+++ | − | +/+++h | ++/+++ | +/++ | +++ | − |
| nucleated fiber cellsf | +/++ | −/(+) | +/++ | ++/+++ | + | ++ | ++ |
| cortical lens fiber cells | ++/+++ | −/(+) | + | +/++ | + | +++ | ++++ |
| nuclear lens fiber cells | ++/+++ | −/(+) | ++ | + | + | ++ | +++ |
| HH stage 39 (ca. 13 days) | |||||||
| capsule | − | − | − | − | − | − | − |
| central anterior epithelial cells | +++ | − | ++/+++h | ++++ | ++ | +++ | −/+ |
| equatorial epithelial cellse | +++ | − | ++/+++h | ++++ | +/++ | +++ | −/+ |
| nucleated lens fiber cellsf | +/++ | −/(+) | ++ | ++++ | + | ++ | ++ |
| cortical lens fiber cells | ++/+++ | −/(+) | + | + | −/+ | +++ | ++++ |
| nuclear lens fiber cells | ++/+++ | −/(+) | ++ | (+) | −/+ | +/++ | + |
| 3-month-old eye (adult)g | |||||||
| capsule | − | − | − | − | − | − | − |
| central epithelial cells | − | − | − | ++++ | ++ | ++ | − |
| equatorial epithelial cellse | − | − | − | ++++ | ++ | ++ | − |
| nucleated fiber cellsf | + | − | (+) | ++++ | + | + | − |
| cortical lens fiber cells | ++ | − | (+) | ++++ | + | − | − |
| nuclear lens fiber cells | ++ | − | − | +→+++ | + | − | +→+++ |
a From [26, 27]; b according to the Hamburger and Hamilton classification [35, 36]; c precursor of central epithelial cells; d precursor of equatorial epithelial cells; e annular pad; f transitional zone; g from [69]; h supranuclear; signal intensity was semiquantitatively grouped into the following categories: −: no staining; (+): very weak but significant staining; +: weak staining; ++: moderate staining; +++: strong staining; ++++: very strong staining; from [29], with permission, and shortened to present data at four instead of eight HH stages.