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. 2021 Apr 29;12:641090. doi: 10.3389/fphar.2021.641090

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Copyright © 2021 Alehaideb, Sheriffdeen and Law.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The separation of caffeine (1) and benzotriazole (2), the internal standard, in saliva extract using isocratic method by HPLC with UV. The retention time for caffeine and benzotriazole is 10.7 and 14.5 min, respectively.