TABLE 5.

The effects of different incubation conditions on the amounts of irreversibly bound radioactivity formed in human liver microsomes.

Incubation mixture	Irreversible bound 8-MOP-derived14C a
	(pmol per mg protein)
Control	3.67 ± 0.39
Boiled microsomes (+90°C)	0.43 ± 0.03 b
Without NADPH (-NADPH)	0.51 ± 0.14 b
Without oxygen (+N2)	2.66 ± 0.44 b
With carbon monoxide (+CO)	2.58 ± 1.06 b

^aResults are expressed as mean ± SD, three independent incubation replicas were performed for each experiment.

^bSignificantly different to control (p ≤ 0.05).