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. 2021 Apr 29;8:649363. doi: 10.3389/fmolb.2021.649363

FIGURE 6.

FIGURE 6

(A) Spearman correlation matrix of CALD1 expression with the 22 tumor-infiltrating immune cell proportions in 85 stage III/IV pMMR CRC samples of GSE39582. (B) Boxplots of CALD1 expression between dMMR and pMMR stage III/IV CRC samples in both GSE39582 (left) and GSE41258 (right) datasets. (C) Histograms of the distribution characteristics of stage III/IV dMMR and pMMR CRC patients in each CMS subgroup. NA, not assigned. (D) Boxplots exhibiting CALD1 expression was significantly and specifically upregulated in the CMS4 subtype in both GSE39582 (left) and GSE41258 (right) datasets. Significant differences between CMS subgroups were indicated as follows: ns, not significant; ***P < 0.001, ****P < 0.0001 (Kruskal–Wallis test followed by Dunn’s tests). (E,F) Correlation heatmap revealed that CALD1 expression was significantly and positively correlated with angiogenesis and TGF-β signaling ssGSEA enrichment scores, immune and stromal scores, gene markers of TAMs (CCL2 and IL10), and M2 macrophages (CD163, VSIG4, and MS4A4A) but was not clear with gene markers of macrophage M1 (IRF5, NOS2, and PTGS2) in the GSE39582 (E) and GSE41258 (F) datasets. pMMR, mismatch-repair-proficient; dMMR, mismatch-repair-deficient; CRC, colorectal cancer; CMS, consensus molecular subtypes; TAMs, tumor-associated macrophages.