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. 2021 Apr 29;8:649363. doi: 10.3389/fmolb.2021.649363

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Copyright © 2021 Zheng, Bai, Wang, Chen, Zhang, Zhu, Liu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CALD1 promotes cell proliferation, migration, and invasion in CRC cells. (A) The protein levels of CALD1 in four kinds of CRC cell lines were measured by Western blot. (B,C) The protein levels of normal, si-NC, and si-CALD1 transfected SW620 (B) and SW480 (C) cells by Western blot; si-CALD1 transfections were performed in duplicate. (D,E) Transwell assays revealed that CALD1 knockdown attenuated SW620 (D) and SW480 (E) cell migration and invasion. (F,G) CCK8 assays were performed to assess cell viability in normal, si-NC, and si-CALD1 transfected SW620 (F) and SW480 (G) cells. *p < 0.05 in comparison with the si-CALD1 group using Kruskal–Wallis one-way analysis of variance (ANOVA). All assays were repeated at least three times.