TABLE 1.
Binding specificity of Siglecs toward N-glycans observed in this study. Recombinant Siglec-Fc chimera proteins were used in this study.
| Siglec | Ligand (preference) | Branch Preference | Strongest N-glycan Binder |
|---|---|---|---|
| Siglec-3 |
(+++);
(+++)
|
α1-3 branch |
|
(++)
|
α1-6 branch | ||
| Siglec-9 |
(+++);
(++)
|
α1-3 branch |
|
(+++);
(++)
|
α1-6 branch | ||
(+++);
(++)
|
α1-6 branch | ||
| Siglec-10 |
(+);
(+++)
|
α1-3 branch |
|
(weak);
(++)
|
α1-6 branch | ||
(weak);
(++)
|
α1-6 branch | ||
| Siglec-F |
(++);
(++)
|
α1-3 branch |
|
Symbols:  GlcNAc  Man  Gal  L-Fuc  Neu5Ac  Neu5Gc | |||