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. 2021 Apr 29;12:664624. doi: 10.3389/fimmu.2021.664624

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Copyright © 2021 Malik, Bjørgen, Nyman, Wessel, Koppang, Dahle and Rimstad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent in situ hybridization of PRV-1, iNOS2 and Arg2 in red focal changes (early phase). (A) Phase contrast image showing a large hemorrhage (star). (B) DNA staining of the cells by DAPI (blue). (C) Presence of a few PRV-1 (green) positive cells in the hemorrhage. (D) iNOS2 (red) specific transcripts detected in a limited number of cells surrounding a peripheral blood vessel. (E) Merged image showing presence of PRV-1 (arrow) but no co-localizing in the M1 macrophage (inset). (F) Arg2 (purple) specific transcripts (M2 type macrophages) were undetected. Scale bar = 100µm.