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. 2021 Apr 29;12:664624. doi: 10.3389/fimmu.2021.664624

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Copyright © 2021 Malik, Bjørgen, Nyman, Wessel, Koppang, Dahle and Rimstad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent in situ hybridization of PRV-1, iNOS2 and Arg2 in red focal changes (intermediate phase). (A) Phase contrast image showing infiltrating cells between myocytes. (B) Nuclei DNA staining of the cells with DAPI (blue). (C) Presence of PRV-1 (green) in infiltrating cells in between myocytes. (D) Presence of iNOS2 (red) in infiltrating cells between myocytes. (E) Merged image showing co-localization (inset) of PRV-1 and iNOS2 (yellow). (F) Arg2 transcripts (purple) were not detected. Scale bar = 100µm.