Table 2.
List of specific primers for genes of interest.
| Genes | Primer | Conc. | Sequence (5’-3’) | Amplicon (bp) | Accession No. |
|---|---|---|---|---|---|
| iNOS2* | F | 400 nM | CATCGGCAGGATTCAGTGGTCCAAT | 135 | XM_014214975.1 |
| R | GGTAATCGCAGACCTTAGGTTTCCTC | ||||
| Arg2* | F | 400 nM | CCTGAAGGACTTGGGTGTCCAGTA | 109 | XM_014190234.1 |
| R | CCGCTGCTTCCTTGACAAGAGGT | ||||
| MHC Class I ( 30 ) | F | 400 nM | CTGCATTGAGTGGCTGAAGA | 175 | AF504022 |
| R | GGTGATCTTGTCCGTCTTTC | ||||
| CD8α ( 31 ) | F | 400 nM | CACTGAGAGAGACGGAAGACG | 174 | AY693393 |
| R | TTCAAAAACCTGCCATAAAGC | ||||
| Granzyme A ( 31 ) | F | 400 nM | GACATCATGCTGCTGAAGTTG | 81 | BT048013 |
| R | TGCCACAGGGACAGGTAACG | ||||
| EF1αb ( 28 ) | F | 500 nM | TGCCCCTCCAGGATGTCTAC | 57 | BG933897 |
| R | CACGGCCCACAGGTACTG |
*Amplification efficiency (E) of newly designed primers were calculated for iNOS2 (E = 0.98) and Arg2 (E = 1.02).