Table 3.
List of probes and corresponding fluorophores used in FISH.
| Probe | Target Region (bp) | Fluorophores | Emission/Excitation Wavelength (nm) | Channel* | |
|---|---|---|---|---|---|
| Target | PRV-L3 | 415–1379 | Opal 520 (FP1487001KT) | 494/525 | C1 |
| iNOS2 | 2–949 | Opal 620 (FP1495001KT) | 588/616 | C2 | |
| Arg2 | 1332–2053 | Opal 690 (FP1497001KT) | 676/694 | C3 | |
| CD8α | 8-1033 | Opal 620 (FP1495001KT) | 588/616 | C2 | |
| GzmA | 3-1088 | Opal 690 (FP1497001KT) | 676/694 | C3 | |
| MHC-I | 2-2321 | Opal 620 (FP1495001KT) | 588/616 | C2 | |
| Control | PPIB | 20–934 | Opal 520 (FP1487001KT) | 494/525 | C1 |
| DapB | 414–862 | Opal 520 (FP1487001KT) | 494/525 | C1 |
*Channels signify the specific labeling of each fluorophore separately for their excitation and emission properties.
Accession numbers; PRV-L3- KY429945; PPIB- NM_001140870; DapB- EF191515, for the other genes the acc. nos. are listed in Table 2 .