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. 2021 Apr 29;9:634151. doi: 10.3389/fcell.2021.634151

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Copyright © 2021 Ding, Cardoso, Yoshimoto and Kobayashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of DN2 phenotype by NICD transduction. (A) E9.5 YS and P-Sp were cultured on DL1-OP9, and CD45⁺c-kit⁺ hematopoietic progenitors were measured on different time points, one of the three independent experiments shown. (B) Representative FACS plots showing DN phenotype on day 12 of YS and P-Sp co-culture on OP9-DL1. (C) CCR9 expression in each DN populations of YS and P-Sp cells co-cultured with DL1-OP9 for 18 days. One of the three independent experiments is depicted. TCRβ and TCRγ expressions in DN cells were also confirmed. (D) The percentage of DN stages and CCR9⁺ cells in each DN population of YS and P-Sp co-culture with OP9DL1, depicted in panel (C). Thymus engraftment of DN3 cells from YS- and P-Sp co-culture with OP9-DL1 cells (E) and their repopulated cell numbers in the recipient thymus (F).