Fig. 5.

AgNPs induces mitochondrial dysfunction in H9c2 cardiac cells. (A) Cells were treated with as-synthesized AgNPs for 24 hr and 48 hr time intervals and analyzed through Mitosox analysis. Representative fluorescent images were detected for confocal microscopy. Red signals represent the time-dependent increase in ROS generation in control and AgNPs treated H9c2 cells; (B) Analysis of ATP production in control and AgNPs treated H9c2 cells. The cells were treated with AgNPs for 24 hr and 48 hr and the ATP content was measured using Cell Titer-Glo Luminescent cell viability assay. The variation in ATP levels was analyzed statistically; (C) H9c2 cells were treated with AgNPs for 24 hr and 48 hr and the expression levels of pAMPK1α marker in control and AgNPs treated cells were measured by Western blot analysis. The variations in the levels were analyzed statistically. Each column represents mean ± SE. (*p < 0.05; **p < 0.01).