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AgNPs induced autophagy in H9c2 cells. Cells were treated with AgNPs for 24 hr and 48 hr and then treated with anti-autophagy proteins. Expression of LC3BII, Atg 5, Beclin1, and LC3BI in control and AgNPs treated H9c2 cells were detected by Western blot analysis. The relative protein levels were quantified by densitometry. The variations in their levels were analyzed statistically. Each column represents mean ± SE. (*p < 0.05; **p < 0.01; ***p < 0.001).
