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. 2021 Mar 17;28(5):2762–2771. doi: 10.1016/j.sjbs.2021.03.021

Fig. 8.

Fig. 8

Amelioration of AgNPs induced cytotoxicity in the presence of NAC. (A) Cells were pre-treated with NAC and then exposed to as-synthesized AgNPs for 48 hr. RT-PCR analysis was done to detect levels of autophagy-related markers where expression of LC3BII and Beclin1 were detected in control and AgNPs treated H9c2 cells in the absence and presence of NAC. A significant decrease in autophagy was detected in AgNPs exposed H9c2 cells pretreated with NAC; (B) BrdU analysis in control and AgNPs treated H9c2 cells in the presence and absence of NAC. Histograms show the data obtained after anti-BrdU staining. AgNPs exposure of NAC treated H9c2 cells caused a significant decrease in DNA fragmentation signifying amelioration of AgNPs toxicity; (C) DNA ladder assay in control and AgNPs treated H9c2 cells in the presence and absence of NAC. Agarose gel electrophoretic image showing the effect of AgNPs on H9c2 cells pretreated with NAC. There was a significant decrease in toxic effects of AgNPs in the presence of NAC. The variations in their levels were analyzed statistically. The variations in their levels were analyzed statistically. Each column represents mean ± SE. (*p < 0.05; **p < 0.01; ***p < 0.001).