Fig. 4.
. Comparison of two CD TCC and taxol (TXA) pore current traces with peptide (AgA(15) and Alm) channel currents. The upper panel shows triangular-shape conductance events induced by TCC and TXL, both at 90 μM. pH = 5.7, V = 100 mV; both traces filtered at 20 kHz. In a high resolution plot (see at the right of the arrow) of a single event only with showing individual points (in Origin 8.5 plot) we observe all points (open circle) with increasing and decreasing, respectively, corresponding values of conductance at both left and right lateral sides of the CD induced triangular conductance events. The lower panel (A) illustrates rectangular-shape conductance events in gA and Alm channels (Ashrafuzzaman et al., 2008). gA channel activity was recorded at V = 200 mV and Alm at V = 150 mV. Here IgA = 29 ± 2, 113 ± 5, 243 ± 9 and 386 ± 10 pA respectively are the discrete current levels 0, 1, 2, 3, …, etc in Alm channel. Traces representing gA and Alm channel activities in phospholipid bilayers were recorded at filter frequencies 2 kHz and 20 kHz, respectively. In (B) the point count plots of the current traces through AgA(15) and Alm channels. The peaks appear at discrete values of conductance for these two channels whereas for the CD pore conducting current traces there is only one delta (type) peak at 0 pA/mV conductance and the points at nonzero values of conductance are distributed all over without showing any specific or discrete conductance values due to the triangular nature of the CD pores (see top-right panel) (Ashrafuzzaman et al., 2011; 2012). The area under the peak at 0 pA/mV conductance of the membrane or 0 pA membrane current is nothing but the total point count proportional to the duration of the time when the membrane hosts no ion pores/channels. The sum of the areas under peaks at various nonzero conductance values (or for CD pores, sum of all points over all nonzero conductance values) is total point count proportional to the duration of time when the membrane conducts currents due to opening of the channels. For the purpose of comparison with previous data (Fig. 3), this Figure is taken from ref. (Ashrafuzzaman et al., 2012).