(A) Mice were weighed from 4 weeks until 12 months old age (n = 24 for one, three, and six months; n = 12 for twelve months) for each genotype. ΛΛΛΛ P < 0.0001, mixed two-way ANOVA of aging effect across different months of ages, ns: nonsignificant (Bonferroni’s multiple comparison test). (B) The rectal temperature of WT (n = 6) and DAT-Ala53 (n = 6) mice was measured and revealed no significant differences (P = 0.4382) between genotypes. (C) Both WT (n = 9) and DAT-Ala53 (n = 9) mice showed no deficits in grip strength behavior and revealed no significant differences between genotypes (P = 0.1156). Data are presented as Mean ± S.D. ns: nonsignificant (unpaired two-tailed Student’s t-test). (D). Sucrose preference for each concentration was presented as a percentage of the volume of sucrose solution consumed to the total volume of solution intake WT (n = 5) and DAT-Ala53 (n = 5). ΛΛΛΛ P < 0.0001, two-way ANOVA for effect of sucrose preference across different concentration of sucrose, ns: nonsignificant effect (Bonferroni’s multiple comparison test).