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. 2021 Apr 29;12:647650. doi: 10.3389/fphar.2021.647650

FIGURE 2.

FIGURE 2

Knockdown of lncRNA MALAT1 downregulated col I, col IV, FN, and LN protein levels and inhibited cell proliferation and invasion in HG-treated HK-2 cells. MALAT1 siRNA and NC siRNA were transfected into cells and then treated with NG and HG, respectively: (A) RT-qPCR was used to determine the expression of MALAT1 in cells. **p < 0.01 vs. NG + NC siRNA group or HG + NC siRNA group. CCK-8 (B) and Transwell assays (C) were used to detect the effects of these two vectors on cell proliferation and invasion. *p < 0.05 vs. NG + NC siRNA group or HG + NC siRNA group, **p < 0.01 vs. NG + NC siRNA group or HG + NC siRNA group. (D and E) ELISA was used to determine the contents of IL-6 and TNF-α in cell lysate when these two vectors were transfected into NG- or HG-treated cells. *p < 0.05 vs. NG + NC siRNA group or HG + NC siRNA group, **p < 0.01 vs. NG + NC siRNA group. (F) Western blotting was used to detect the expression of col Ⅰ, col Ⅳ, FN, and LN in NG- or HG-treated cells after transfection with these two vectors. The histogram in (G–J) shows the densitometric analysis of the blots (col I, col IV, FN, and LN) normalized to GAPDH. *p < 0.05 vs. NG + NC siRNA group or HG + NC siRNA group, **p < 0.01 vs. NG + NC siRNA group.