FIGURE 3.

Overexpression of lncRNA MALAT1 upregulated col I, col IV, FN, and LN protein levels in HG-treated HK-2 cells. Cells were transfected with pcDNA-MALAT1 (MALAT1) and pcDNA3.1 (vector) and then treated with NG or HG, respectively: (A) RT-qPCR was used to determine the expression of MALAT1 in cells transfected with these two vectors. **p < 0.01 vs. NG + vector group or HG + vector group. (B) Expression of col I, col IV, FN, and LN in cells transfected with these two vectors was detected by Western blotting. The histogram in (C–F) shows the densitometric analysis of the bands (col I, col IV, FN, and LN) normalized to GAPDH. **p < 0.01 vs. NG + vector group or HG + vector group.