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. 2021 Apr 29;12:647650. doi: 10.3389/fphar.2021.647650

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Copyright © 2021 Huang, Zhang and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL6ST was a target of miR-2355-3p. (A) StarBase (http://starbase.sysu.edu.cn/) was used to predict the binding between IL6ST and miR-2355-3p. (B) Luciferase reporter gene assay in HEK293T cells was used to verify the binding between IL6ST and miR-2355-3p. **p < 0.01 vs. NC mimic group. (C and D) The expression of IL6ST was detected by RT-qPCR and Western blotting in NG- or HG-treated cells transfected with miR-2355-3p mimic or NC mimic. **p < 0.01 vs. NG + NC mimic group or HG + NC mimic group. The histogram in (E) presents the densitometric analysis of the blots (IL6ST) normalized to GAPDH. **p < 0.01 vs. NG + NC mimic group or HG + NC mimic group.