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. 2021 Apr 29;12:647650. doi: 10.3389/fphar.2021.647650

FIGURE 6.

FIGURE 6

IL6ST knockdown downregulated col I, col IV, FN, and LN protein levels and blocked the STAT3/NF-kB signaling pathway in HG-treated HK-2 cells. (A) Western blotting was used to detect the IL6ST level in cells treated by HG for 0, 24, 48, and 72 h. The histogram in (B) shows the densitometric analysis of the blots (IL6ST) normalized to GAPDH. **p < 0.01 vs. 0 h group. (C) col I, col IV, FN, LN, and IL6ST protein levels in cells transfected with IL6ST siRNA or NC siRNA were detected by Western blotting. (D) Densitometric analysis of the blots (col I, col IV, FN, LN, and IL6ST) normalized to GAPDH. **p < 0.01 vs. NG + NC siRNA or HG + NC siRNA group. (E) Western blotting was used to detect the STAT3 phosphorylation level, total STAT3, and NF-kB p65 protein levels in NG- or HG-treated cells. The histogram in (F) presents the densitometric analysis of the blots (p-STAT3/STAT3 and NF-kB p65) normalized to GAPDH. **p < 0.01 vs. NG + NC siRNA group or HG + NC siRNA group.