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. 2021 May 11;218(7):e20202477. doi: 10.1084/jem.20202477

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© 2021 Nguyen et al.

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Figure 6. — Loss of Cbl-b in W131AOTII mice significantly altered gene expression profile. (A) RNA sequencing revealed heatmap analysis of gene expression of peripheral naive (CD44^low CD62L⁺) CD25⁻Vα2⁺ CD4 T cells (n = 3–12 mice/group). (B) Principal component analysis of CD25⁻Vα2⁺ peripheral naive CD4 T cells from the indicated mouse strains. (C) Top five signaling pathways enriched in peripheral naive Cbl-b^−/−W131AOTII T cells compared with W131AOTII T cells. (D) MA plot revealed the top 20 genes whose expressions were highly differentiated in peripheral Cbl-b^−/−W131AOTII compared with those from W131AOTII mice. (E–H) RNA sequencing revealed gene expression of anergy-associated genes (E), Socs genes (F), Ubc (G), and anti-apoptotic Bcl2 (H) in peripheral naive CD25⁻Vα2⁺CD4⁺ from the indicated mice. The expression values were normalized by fragments per kilobase of transcript per million reads (FPKM). FDR-corrected *, P < 0.05; **, P < 0.005; ***, P < 0.0005. GO, gene ontology; ID, identification.