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. 2021 May 11;218(7):e20202477. doi: 10.1084/jem.20202477

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© 2021 Nguyen et al.

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Figure S3. — In contrast to CD4SP thymocytes, tolerized gene signatures were strongly enriched in peripheral W131AOTII T cells. (A) Volcano plot revealed expression of anergy-associated genes, T reg cell–associated genes, inhibitory genes influencing cytokines and TCR signaling, and exhausted genes in peripheral naive CD25⁻Vα2⁺CD4⁺ T cells from W131AOTII compared with those cells from OTII mice (n = 3–12 mice/group). (B) RNA sequencing revealed expression of exhausted genes in peripheral naive CD25⁻Vα2⁺CD4⁺ T cells. (C) Volcano plot revealed expression of anergy-associated genes, T reg cell–associated genes, inhibitory genes influencing cytokines and TCR signaling, and exhausted genes in CD25⁻Vα2⁺CD4SP thymocytes from W131AOTII compared with those cells from OTII mice. NA, not applicable. (D) RNA sequencing revealed expression of exhausted genes in CD25⁻Vα2⁺ CD4SP thymocytes from OTII versus W131AOTII mice. The expression values were normalized by fragments per kilobase of transcript per million reads (FPKM) of W131AOTII T cells versus OTII T cells. FDR-corrected *, P < 0.05; **, P < 0.005; ***, P < 0.0005.