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. 2021 May 11;218(7):e20202477. doi: 10.1084/jem.20202477

Figure S4.

Figure S4.

Deletion of Cbl-b, Grail, or PD-1 in W131AOTII mice did not rescue anergic phenotypes in W131AOTII mice. (A) Representative plots showing expression of CD4 and CD8 in total thymocytes from indicated mice. (B–D) Bar graphs show frequencies of double-negative (B; DN), DP (C), and CD4SP thymocytes (D) in indicated mice. (E) Bar graph shows the frequencies of thymic T reg cells (Foxp3+) of Vα2+ CD4SP thymocytes. (F) FACS plots showing PD-1 staining in peripheral Vα2+ CD4 T cells from OTII and W131AOTII mice. (G) MFI of PD-1 expression. (H) Histograms comparing expression of TCRβ, CD3, and Vα2 in peripheral naive Vα2+CD4+ T cells from indicated mice. (I) Bar graphs indicating MFI ± SD for TCRβ, CD3, and Vα2 in peripheral naive Vα2+CD4+ T cells from indicated mice. Data in A–E are combined from five independent experiments (n = 3–17 mice/group). Data in F–I are presentative of two independent experiments (n = 3 or 4 mice/group). Two-tailed Student’s t test was performed. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.